1.
Chinese Journal of Immunology
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-547789
ABSTRACT
Objective:To examine Toll-like receptor expression pattern in monocytes-derived macrophages by Lipopolysaccharid(LPS) stimulation.Methods:Jugular venous blood was collected from three Japanese calves,and then the peripheral blood mononuclear cells(PBMC),were isolated,and cultured for 7 days to collect monocytes-derived macrophages in Repcell and the cells were stimulated with LPS for 24 h to analyze the mRNA expression pattern of TLR in monocytes-derived macrophages.Results:The mRNA expressions of IL-6,TNF-? and IL-1? were significantly increased after LPS stimulation(P